科研项目：

       先后主持过国家自然科学基金重点（1项）、重大研究计划（1项）和面上项目（5项），以及科技部973项目子课题（1项）。

       在研项目：

     （1）组蛋白H2B单泛素化修饰对植物内源生长素稳态的影响和作用机理（国家自然基金面上）；
（2）CKRW2影响PIN2细胞定位的机理研究（甘肃省基金）；
（3）NatB介导的N-α-乙酰化影响ACC氧化酶蛋白稳定性和乙烯生物合成的机理（重点实验室开放基金）。

研究成果：

长期系统研究植物生长素，独立于国际上3家实验室发现生长素生物合成IPA途径突变体taa1/ckrc1；独立提出和证明了细胞分裂素通过促进生长素合成并抑制运输的作用机理调控植物发育[Plant J., 2011, 66(3): 516-527]。随后，在生长素合成的转录调控[Sci. Rep., 2016, 6, 36866]、表观遗传调控[Commun Biol., 2021, 4(1): 206]和翻译后蛋白修饰调控[Commun Biol., 2022, 5(1): 1410]取得了重要进展。

       此外更早期，1989年攻克植物细胞培养世界性难题，在小麦原生质体培养中实现再生细胞直接形成体细胞胚和再生植株 [科学通报(中/英文版1990)，中国科学（中/英文版1990/1991)]；随后又成功进行了小麦遗传转化 [科学通报(中/英文版1993; 2000)]。2004年发表胞间桥/通道功能假说[J Theor Biol., 2004, 229: 139-146]，引起国际关注，被Cell、PNAS、Trends Mol Med、Biol Rev、Cold Spring Harb Perspect Biol和Dev Biol等期刊及相关专著所引用。

可同时招收硕士及博士研究生。

Research Interests

1、 生长素合成、运输、代谢和信号调控 Auxin biosynthesis, metabolism, polar transport and signalling

       生长素是重要的植物激素，在调控植物生长发育和信号响应中起关键作用。我们以模式植物拟南芥和二穗短柄草为材料，综合运用生物化学、遗传学和分子细胞生物学等最新技术研究生长素的生物合成、极性运输、代谢及信号转导的分子机制、功能和应用。

       Auxin is one of the most important plant hormones regulating plant growth and development, such as cell division, cell differentiation, apical dominance, flowering, senescence, and tropism. Plants maintain auxin homeostasis by regulating its synthesis, metabolism, and polar transport. We use model plants Arabidopsis and B. distachyon, with modern biochemical, molecular genetic and cell biological techniques to studies auxin biosynthesis, polar transport, metabolism and signalling and their functions in plant growth and development.

2. 生殖细胞发育 Germ cell development

       有性生殖涉及减数分裂、配子发育、受精和胚胎发生等过程，受到基因精细调控并需要细胞间的通讯和相互协调。植物方面我们主要用拟南芥为材料分离突变体，用分子生物学手段研究大小孢子发生及其配子体发育的分子机理；动物方面则以小鼠为材料研究胞间桥/通道在生殖细胞发育过程中的功能和细胞生物学机理。
Sexual reproduction occurs via meiosis, gamete formation, firtilization and embryogenesis under strict genetic control with highly coordination between cells. We use the current powerful tools in biochemstry, genetics, cell and molecular biology to study the related molecular mechanisms through isolating mutants in Arabidopsis and cloning the corresponding genes in plants.  Moreover, we use mouse as a model to investigate the biological significance and cellular mechanisms of intercellular bridges in animal germ cell development.

部分发表论文（按年份顺序排列；*通讯联系人）

• 郭光沁、夏光敏、李忠谊、陈惠民，1990，小麦原生质体培养体细胞胚直接发生再生植株。科学通报 35(6): 480.
• Guo GQ, Xia GM, Li ZY, Chen HM, 1990, Direct somatic embryogenesis and plant regeneration in protoplast culture of wheat. Chin Sci B (Sci Bull) 35(17): 1495-6.
• 郭光沁、夏光敏、李忠谊、陈惠民，1990，小麦原生质体再生细胞直接形成体细胞胚和再生植株。中国科学B辑 20(9): 970-4。
• Guo GQ, Xia GM, Li ZY, Chen HM, 1991, Direct somatic embryogenesis and plant regeneration from protoplast-derived cells of wheat. Sci in China (ser B) 34(4): 438-45.
• 郭光沁、许智宏、卫志明、陈惠民，1993，用PEG法向小麦原生质体导入外源基因获得转基因植株。科学通报 38(13): 1227-31。
• Guo GQ, Xu ZH, Wei ZM, Chen HM, 1993, Trangenic plants obtained from wheat protoplasts transformed by PEG-mediated direct gene transfer. Chin Sci B (Sci Bull) 38(24): 2072-8.
• Guo GQ, Maiwald F, Lorenzen P & Steinbiss HH, 1998, Factors influencing T-DNA transfer into wheat and barley cells by Agrobacterium tumefaciens. Cereal Res Comm 26: 15-22.
• 李卫、郭光沁*、郑国锠，2000，根癌农杆菌介导遗传转化研究的若干新进展。科学通报 45(8): 798-807。
• Li W, Guo GQ*, Zheng GC, 2000, Agrobacterium-mediated transformation: state of the art and future prospect. Chin Sci B (Sci Bull) 45(17): 1537-46.
• Guo GQ*, Zheng GC, 2004, Hypotheses for the functions of intercellular bridges in male germ cell development and its cellular mechanisms. J Theor Biol 229: 139-146.
• Wang QF, Li W, Yang Hai, Liu YL, Cao HH, Dornmayr-Pfaffenhuemer M, Stan-Lotter H, Guo GQ*, 2007, Halococcus qingdaonensis sp. nov., a halophilic archaeon isolated from a crude sea-salt sample. Int J Syst Evol Microbiol 57: 600-604.
• Liu Y, Yan Z, Chen N, Di X, Huang J, Guo G*. 2010. Development and function of central cell in angiosperm female gametophyte. Genesis (Dev Genet) 48: 466-478.
• Zhou ZY, Zhang CG, Wu L, Zhang CG, Chai J, Wang M, Jha A, Jia PF, Cui SJ, Yang M, Chen R, Guo GQ*. 2011. Functional characterization of the CKRC1/TAA1 gene and dissection of hormonal actions in the Arabidopsis root. Plant J 66: 516–527.
• Wang L, Sun C, Wang ZH, Guo GQ*（联系人）. 2012. Mechanism of apoptotosis induced by ortho-topolin riboside in human hepatoma cell line SMMC-7721. Food Chem Toxico 50, 1962-1968.
• Wu L, Pan L, Di DW, Wang L, Wang M, Lu CK, Wei SD, Zhang L, Zhang TZ, Amakorová P, Mrvková M, Novák O, Guo GQ*. 2015. Forward genetic screen for auxin-deficient mutants by cytokinin. Sci Rep 5, 11923; doi: 10.1038/srep11923.
• Wu L, Zhou ZY, Zhang CG, Chai J, Zhou Q, Wang L, Hirnerová E, Mrvková M, Novák O, Guo GQ*. 2015. Functional roles of three cutin biosynthetic acyltransferases in cytokinin responses and skotomorphogenesis. PLoS One 10(3): e0121943; doi: 10.1371/journal.pone.0121943.
• Di DW, Zhang C, Luo P, An CW, Guo GQ. 2015. The biosynthesis of auxin: How many paths truly lead to IAA? Plant Growth Regulation 78, 275-285.
• Di DW, Zhang C, Guo GQ. 2015. Involvement of secondary messengers and small organic molecules in auxin perception and signaling. Plant Cell Rep 34(6):895-904.
• Zhao L, Liu P, Guo G*, Wang L*. 2015. Combination of cytokinin and auxin induces apoptosis, cell cycle progression arrest and blockage of the Akt pathway in HeLa cells. Mol Med Rep 12: 719-727.
• Di DW, Wu L, Zhang L, An CW, Zhang TZ, Luo P, Gao HH, Kriechbaumer V, Guo GQ*. 2016. Functional roles of Arabidopsis CKRC2/YUCCA8 gene and the involvement of PIF4 in the regulation of auxin biosynthesis by cytokinin. Sci Rep 6, 36866; doi: 10.1038/srep36866.
• Shah JN, Guo GQ*, Krishnan A*, Ramesh M, Katari NK, Shahbaaz M, Abdellattif MH, Singh SK, Dua K. 2021. Peptides-based therapeutics: Emerging potential therapeutic agents for COVID-19 [published online ahead of print, 2021 Oct 8]. Therapie S0040-5957(21)00204-3. doi:10.1016/j.therap.2021.09.007.
• Liu HQ, Zou YJ, Li XF, Wu L, Guo GQ*. 2021. Stablization of ACOs by NatB mediated N-terminal acetylation is required for ethylene homeostasis. BMC Plant Biol 21(1):320. doi: 10.1186/s12870-021-03090-7.
• Zhang L, Luo P, Bai J, Wu L, Di DW, Liu HQ, Li JJ, Liu YL, Khaskheli AJ, Zhao CM, Guo GQ*. 2021. Function of histone H2B monoubiquitination in transcriptional regulation of auxin biosynthesis in Arabidopsis. Commun Biol 4(1):206. doi: 10.1038/s42003-021-01733-x.
• Wu L, Wang JL, Li XF, Guo GQ*. 2021. Cytokinin-Controlled Gradient Distribution of Auxin in Arabidopsis Root Tip. Int J Mol Sci 22(8):3874. doi: 10.3390/ijms22083874.
• Liu HQ, Pu ZX, Di DW, Zou YJ, Guo YM, Wang JL, Zhang L, Tian P, Fei QH, Li XF, Khaskheli AJ, Wu L, Guo GQ*. 2022. Significance of NatB-mediated N-terminal acetylation of auxin biosynthetic enzymes in maintaining auxin homeostasis in Arabidopsis thaliana. Commun Biol 5, 1410. doi: 10.1038/s42003-022-04313-9.
  
• Zhou Y, Zhang J, Lin H, Guo GQ, Guo Y. 2010. MORPHEUS’ MOLECULE1 is required to prevent aberrant RNA transcriptional read-through in Arabidopsis. Plant Physiol 154: 1272–1280.
• Zhang CG, Guo HH, Zhang J, Guo GQ, Schumaker K, Guo Y. 2010. Arabidopsis CSAat1A and CSAat1B proteins form a complex with CULLIN4 and DDB1A and regulate the response to UV irradiation. Plant Cell 22: 2353-2369.